Update MPV_3000.primer.bed to include strand information for line 1#1
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mattloose wants to merge 1 commit intoquick-lab:masterfrom
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Update MPV_3000.primer.bed to include strand information for line 1#1mattloose wants to merge 1 commit intoquick-lab:masterfrom
mattloose wants to merge 1 commit intoquick-lab:masterfrom
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The new primer added at line 1 is missing strand information and so breaks the bed format - here we've added in the strand information on line 1 - but you would now have two primers with the same name and different strands - not sure if that breaks any downstream tools.
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I had wondered about that - the issue is how to represent a single primer that amplifies two regions of the genome. We could give it an underscore at the end or something? Any suggestions? |
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I don't really know - if people use this file to order primers then the duplication of a primer is confusing. In this case, the primer is acting as a left primer at the start - you could just name it as _Left instead of _Right - but that might lead to people purchasing one extra primer? |
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Yeah OK will fix now |
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The new primer added at line 1 is missing strand information and so breaks the bed format - here we've added in the strand information on line 1 - but you would now have two primers with the same name and different strands - not sure if that breaks any downstream tools.