diff --git a/src/spatialdata_plot/pl/basic.py b/src/spatialdata_plot/pl/basic.py
index 2b7401c1..3567efa7 100644
--- a/src/spatialdata_plot/pl/basic.py
+++ b/src/spatialdata_plot/pl/basic.py
@@ -950,6 +950,10 @@ def show(
             if not all(isinstance(t, str) for t in title):
                 raise TypeError("All titles must be strings.")
 
+        # Track whether the caller supplied their own axes so we can skip
+        # plt.show() later (ax is reassigned inside the rendering loop).
+        user_supplied_ax = ax is not None
+
         # get original axis extent for later comparison
         ax_x_min, ax_x_max = (np.inf, -np.inf)
         ax_y_min, ax_y_max = (np.inf, -np.inf)
@@ -1273,8 +1277,11 @@ def _draw_colorbar(
         # Default (show=None): display in non-interactive mode (scripts), suppress in interactive
         # sessions. We check both sys.ps1 (standard REPL) and matplotlib.is_interactive()
         # (covers IPython, Jupyter, plt.ion(), and IDE consoles like PyCharm).
+        # When the user supplies their own axes, they manage the figure lifecycle, so we
+        # default to not calling plt.show(). This allows multiple .pl.show(ax=...) calls
+        # to accumulate content on the same axes (see #362, #71).
         if show is None:
-            show = not hasattr(sys, "ps1") and not matplotlib.is_interactive()
+            show = False if user_supplied_ax else (not hasattr(sys, "ps1") and not matplotlib.is_interactive())
         if show:
             plt.show()
         return (fig_params.ax if fig_params.axs is None else fig_params.axs) if return_ax else None  # shuts up ruff
diff --git a/tests/pl/test_show.py b/tests/pl/test_show.py
index 2b7e444d..a4033d2b 100644
--- a/tests/pl/test_show.py
+++ b/tests/pl/test_show.py
@@ -1,4 +1,7 @@
+from unittest.mock import patch
+
 import matplotlib
+import matplotlib.pyplot as plt
 import scanpy as sc
 from spatialdata import SpatialData
 
@@ -21,3 +24,19 @@
 class TestShow(PlotTester, metaclass=PlotTesterMeta):
     def test_plot_pad_extent_adds_padding(self, sdata_blobs: SpatialData):
         sdata_blobs.pl.render_images(element="blobs_image").pl.show(pad_extent=100)
+
+    def test_no_plt_show_when_ax_provided(self, sdata_blobs: SpatialData):
+        """plt.show() must not be called when the user supplies ax= (regression for #362)."""
+        _, ax = plt.subplots()
+        with patch("spatialdata_plot.pl.basic.plt.show") as mock_show:
+            sdata_blobs.pl.render_images(element="blobs_image").pl.show(ax=ax)
+            mock_show.assert_not_called()
+        plt.close("all")
+
+    def test_plt_show_when_ax_provided_and_show_true(self, sdata_blobs: SpatialData):
+        """Explicit show=True still calls plt.show() even with ax=."""
+        _, ax = plt.subplots()
+        with patch("spatialdata_plot.pl.basic.plt.show") as mock_show:
+            sdata_blobs.pl.render_images(element="blobs_image").pl.show(ax=ax, show=True)
+            mock_show.assert_called_once()
+        plt.close("all")