diff --git a/Lib/profiling/sampling/gecko_collector.py b/Lib/profiling/sampling/gecko_collector.py
index 2de8cce387e7f26..bc6ce6187b8846d 100644
--- a/Lib/profiling/sampling/gecko_collector.py
+++ b/Lib/profiling/sampling/gecko_collector.py
@@ -617,7 +617,7 @@ def _get_or_create_func(self, thread_data, filename, funcname, lineno):
         func_resources.append(resource_idx)
 
         if is_python:
-            filename_idx = self._intern_string(os.path.basename(filename))
+            filename_idx = self._intern_string(filename)
             func_filenames.append(filename_idx)
             func_line_numbers.append(lineno)
         else:
diff --git a/Lib/test/test_profiling/test_sampling_profiler/test_collectors.py b/Lib/test/test_profiling/test_sampling_profiler/test_collectors.py
index d4ba2df5044559f..b6c435b58ecfa83 100644
--- a/Lib/test/test_profiling/test_sampling_profiler/test_collectors.py
+++ b/Lib/test/test_profiling/test_sampling_profiler/test_collectors.py
@@ -741,6 +741,51 @@ def test_gecko_collector_basic(self):
         self.assertGreater(stack_table["length"], 0)
         self.assertGreater(len(stack_table["frame"]), 0)
 
+    def test_gecko_collector_preserves_source_file_paths(self):
+        """Test that GeckoCollector preserves source file paths and distinguishes
+        files with the same basename in different directories."""
+        collector = GeckoCollector(1000)
+        file_a = os.path.join("pkg_a", "__init__.py")
+        file_b = os.path.join("pkg_b", "__init__.py")
+
+        test_frames = [
+            MockInterpreterInfo(
+                0,
+                [
+                    MockThreadInfo(
+                        1,
+                        [
+                            MockFrameInfo(file_a, 10, "func_a"),
+                            MockFrameInfo(file_b, 20, "func_b"),
+                        ],
+                        status=THREAD_STATUS_MAIN_THREAD,
+                    )
+                ],
+            )
+        ]
+        collector.collect(test_frames)
+        profile_data = collector._build_profile()
+
+        string_array = profile_data["shared"]["stringArray"]
+        func_table = profile_data["threads"][0]["funcTable"]
+
+        self.assertEqual(func_table["length"], 2)
+
+        funcs_by_name = {
+            string_array[func_table["name"][i]]: (
+                func_table["fileName"][i],
+                string_array[func_table["fileName"][i]],
+            )
+            for i in range(func_table["length"])
+        }
+
+        file_idx_a, resolved_file_a = funcs_by_name["func_a"]
+        file_idx_b, resolved_file_b = funcs_by_name["func_b"]
+
+        self.assertEqual(resolved_file_a, file_a)
+        self.assertEqual(resolved_file_b, file_b)
+        self.assertNotEqual(file_idx_a, file_idx_b)
+
     def test_gecko_collector_async_aware(self):
         collector = GeckoCollector(1000)
 
diff --git a/Misc/NEWS.d/next/Library/2026-10-30-23-05-00.gh-issue-160976.GeCk0P.rst b/Misc/NEWS.d/next/Library/2026-10-30-23-05-00.gh-issue-160976.GeCk0P.rst
new file mode 100644
index 000000000000000..1da8a1f97e6f98f
--- /dev/null
+++ b/Misc/NEWS.d/next/Library/2026-10-30-23-05-00.gh-issue-160976.GeCk0P.rst
@@ -0,0 +1 @@
+The Gecko reporter (``--gecko``) in ``profiling.sampling`` now records full source file paths instead of only basenames.