diff --git a/changelog/11281.bugfix.rst b/changelog/11281.bugfix.rst
new file mode 100644
index 00000000000..e713087e0f1
--- /dev/null
+++ b/changelog/11281.bugfix.rst
@@ -0,0 +1,6 @@
+Fixed fixture discovery to preserve definition order instead of using alphabetical sorting.
+
+This ensures that fixtures are processed in the order they appear in source code,
+which is important for autouse fixtures and fixture overriding with the ``name`` parameter.
+Previously, using ``dir()`` for fixture discovery caused alphabetical sorting, leading to
+unexpected behavior when fixtures with the same name were defined at different scopes.
diff --git a/src/_pytest/fixtures.py b/src/_pytest/fixtures.py
index 84f90f946be..2024fe678a6 100644
--- a/src/_pytest/fixtures.py
+++ b/src/_pytest/fixtures.py
@@ -1869,35 +1869,60 @@ def parsefactories(
             holderobj_tp = holderobj
 
         self._holderobjseen.add(holderobj)
-        for name in dir(holderobj):
-            # The attribute can be an arbitrary descriptor, so the attribute
-            # access below can raise. safe_getattr() ignores such exceptions.
-            obj_ub = safe_getattr(holderobj_tp, name, None)
-            if type(obj_ub) is FixtureFunctionDefinition:
-                marker = obj_ub._fixture_function_marker
-                if marker.name:
-                    fixture_name = marker.name
-                else:
-                    fixture_name = name
-
-                # OK we know it is a fixture -- now safe to look up on the _instance_.
-                try:
-                    obj = getattr(holderobj, name)
-                # if the fixture is named in the decorator we cannot find it in the module
-                except AttributeError:
-                    obj = obj_ub
-
-                func = obj._get_wrapped_function()
-
-                self._register_fixture(
-                    name=fixture_name,
-                    nodeid=nodeid,
-                    func=func,
-                    scope=marker.scope,
-                    params=marker.params,
-                    ids=marker.ids,
-                    autouse=marker.autouse,
-                )
+
+        # Use __dict__ to preserve definition order instead of dir() which sorts.
+        # This ensures fixtures are processed in their definition order, which is
+        # important for autouse fixtures and fixture overriding (#11281, #12952).
+        #
+        # For modules: module.__dict__ contains all module-level names.
+        # For classes: walk the MRO to get all class and base class attributes.
+        # For instances (e.g. unittest TestCase instances): walk the class MRO,
+        # since fixtures are defined on the class, not the instance.
+        dicts: list[Mapping[str, Any]]
+        if isinstance(holderobj, types.ModuleType):
+            dicts = [holderobj.__dict__]
+        elif safe_isclass(holderobj):
+            assert isinstance(holderobj, type)
+            dicts = [cls.__dict__ for cls in holderobj.__mro__]
+        else:
+            # Instance: walk the class hierarchy.
+            dicts = [cls.__dict__ for cls in type(holderobj).__mro__]
+
+        seen: set[str] = set()
+        for dic in dicts:
+            for name in dic:
+                if name in seen:
+                    continue
+                seen.add(name)
+
+                # The attribute can be an arbitrary descriptor, so the attribute
+                # access below can raise. safe_getattr() ignores such exceptions.
+                obj_ub = safe_getattr(holderobj_tp, name, None)
+                if type(obj_ub) is FixtureFunctionDefinition:
+                    marker = obj_ub._fixture_function_marker
+                    if marker.name:
+                        fixture_name = marker.name
+                    else:
+                        fixture_name = name
+
+                    # OK we know it is a fixture -- now safe to look up on the _instance_.
+                    try:
+                        obj = getattr(holderobj, name)
+                    # if the fixture is named in the decorator we cannot find it in the module
+                    except AttributeError:
+                        obj = obj_ub
+
+                    func = obj._get_wrapped_function()
+
+                    self._register_fixture(
+                        name=fixture_name,
+                        nodeid=nodeid,
+                        func=func,
+                        scope=marker.scope,
+                        params=marker.params,
+                        ids=marker.ids,
+                        autouse=marker.autouse,
+                    )
 
     def getfixturedefs(
         self, argname: str, node: nodes.Node
diff --git a/testing/python/fixtures.py b/testing/python/fixtures.py
index 8b9e3fbb0a5..e3ad79b2556 100644
--- a/testing/python/fixtures.py
+++ b/testing/python/fixtures.py
@@ -5399,3 +5399,65 @@ def test_it(request, fix1):
     )
     result = pytester.runpytest("-v")
     result.assert_outcomes(passed=1)
+
+
+def test_autouse_fixtures_definition_order_preserved(pytester: Pytester) -> None:
+    """
+    Test that fixture discovery uses definition order instead of alphabetical
+    sorting from dir(). When fixtures have different names, they should be
+    discovered and registered in their definition order, which ensures
+    higher-scoped fixtures execute before lower-scoped ones.
+
+    Regression test for https://github.com/pytest-dev/pytest/issues/11281
+    """
+    pytester.makepyfile(
+        """
+        import pytest
+
+        call_order = []
+
+        @pytest.fixture(scope='module', autouse=True)
+        def module_setup():
+            call_order.append("MODULE")
+
+        class TestFoo:
+            @pytest.fixture(scope='class', autouse=True)
+            def class_setup(self):
+                call_order.append("CLASS")
+
+            def test_in_class(self):
+                # Module-scoped fixture runs first, then class-scoped.
+                assert call_order == ["MODULE", "CLASS"]
+        """
+    )
+    result = pytester.runpytest("-v")
+    result.assert_outcomes(passed=1)
+
+
+def test_fixture_override_with_name_kwarg_respects_definition_order(
+    pytester: Pytester,
+) -> None:
+    """
+    Test that fixture override using name kwarg respects definition order.
+
+    Related to https://github.com/pytest-dev/pytest/issues/12952
+    """
+    pytester.makepyfile(
+        """
+        import pytest
+
+        @pytest.fixture()
+        def f1():
+            return 1
+
+        @pytest.fixture(name="f1")
+        def f2():
+            return 2
+
+        def test_override(f1):
+            # Later definition should override
+            assert f1 == 2
+        """
+    )
+    result = pytester.runpytest("-v")
+    result.assert_outcomes(passed=1)