diff --git a/changelog/14080.bugfix.rst b/changelog/14080.bugfix.rst
new file mode 100644
index 00000000000..1140b4716db
--- /dev/null
+++ b/changelog/14080.bugfix.rst
@@ -0,0 +1 @@
+fix missing type annotations on ``Pytester.makepyfile`` and ``Pytester.maketxtfile`` methods.
diff --git a/src/_pytest/pytester.py b/src/_pytest/pytester.py
index 1cd5f05dd7e..2313b45ce02 100644
--- a/src/_pytest/pytester.py
+++ b/src/_pytest/pytester.py
@@ -10,6 +10,7 @@
 from collections.abc import Callable
 from collections.abc import Generator
 from collections.abc import Iterable
+from collections.abc import Mapping
 from collections.abc import Sequence
 import contextlib
 from fnmatch import fnmatch
@@ -645,6 +646,9 @@ def restore(self) -> None:
         sys.path[:], sys.meta_path[:] = self.__saved
 
 
+_FileContent = tuple[str | bytes, ...] | list[str | bytes] | str | bytes
+
+
 @final
 class Pytester:
     """
@@ -756,7 +760,7 @@ def _makefile(
         self,
         ext: str,
         lines: Sequence[Any | bytes],
-        files: dict[str, str],
+        files: Mapping[str, _FileContent],
         encoding: str = "utf-8",
     ) -> Path:
         items = list(files.items())
@@ -862,7 +866,7 @@ def makepyprojecttoml(self, source: str) -> Path:
         """
         return self.makefile(".toml", pyproject=source)
 
-    def makepyfile(self, *args, **kwargs) -> Path:
+    def makepyfile(self, *args: _FileContent, **kwargs: _FileContent) -> Path:
         r"""Shortcut for .makefile() with a .py extension.
 
         Defaults to the test name with a '.py' extension, e.g test_foobar.py, overwriting
@@ -882,7 +886,7 @@ def test_something(pytester):
         """
         return self._makefile(".py", args, kwargs)
 
-    def maketxtfile(self, *args, **kwargs) -> Path:
+    def maketxtfile(self, *args: _FileContent, **kwargs: _FileContent) -> Path:
         r"""Shortcut for .makefile() with a .txt extension.
 
         Defaults to the test name with a '.txt' extension, e.g test_foobar.txt, overwriting