Hello,
Recently, I've been analyzing RNA modification data from DRS (Direct RNA Sequencing) and would like to extract information for a specific type of RNA modification individually. I used the following code to extract modification information of inosine , but the results do not include inosine modifications.
${modkit} pileup --ref ${ref} --preload-references --modified-bases A:17596 --log-filepath ${output}/modkit_${name}.log --header --threads ${thread} ${workdir}/${bam} ${output}/test.pileup.bed
Here is the output:
And here is the information of modification tags:
Then I tried extracting all available modification types, and the results were normal.
Why is this happening? Looking forward to the developer's response. Thank you very much!
Hello,
Recently, I've been analyzing RNA modification data from DRS (Direct RNA Sequencing) and would like to extract information for a specific type of RNA modification individually. I used the following code to extract modification information of inosine , but the results do not include inosine modifications.
${modkit} pileup --ref ${ref} --preload-references --modified-bases A:17596 --log-filepath ${output}/modkit_${name}.log --header --threads ${thread} ${workdir}/${bam} ${output}/test.pileup.bedHere is the output:
And here is the information of modification tags:
Then I tried extracting all available modification types, and the results were normal.
Why is this happening? Looking forward to the developer's response. Thank you very much!