diff --git a/core/api.txt b/core/api.txt
index 745d82786af..edc993c3af6 100644
--- a/core/api.txt
+++ b/core/api.txt
@@ -974,6 +974,8 @@ ion-item-option,css-prop,--background,ios
 ion-item-option,css-prop,--background,md
 ion-item-option,css-prop,--color,ios
 ion-item-option,css-prop,--color,md
+ion-item-option,part,container
+ion-item-option,part,inner
 ion-item-option,part,native
 
 ion-item-options,none
diff --git a/core/src/components/item-option/item-option.tsx b/core/src/components/item-option/item-option.tsx
index 81bee8b8fef..7ea1bc2fd4b 100644
--- a/core/src/components/item-option/item-option.tsx
+++ b/core/src/components/item-option/item-option.tsx
@@ -17,6 +17,8 @@ import type { Color } from '../../interface';
  * @slot end - Content is placed to the right of the option text in LTR, and to the left in RTL.
  *
  * @part native - The native HTML button or anchor element that wraps all child elements.
+ * @part inner - The inner container element that wraps the option content.
+ * @part container - The container element that wraps the start, icon-only, default, and end slots.
  */
 @Component({
   tag: 'ion-item-option',
@@ -109,9 +111,9 @@ export class ItemOption implements ComponentInterface, AnchorInterface, ButtonIn
         })}
       >
         <TagType {...attrs} class="button-native" part="native" disabled={disabled}>
-          <span class="button-inner">
+          <span class="button-inner" part="inner">
             <slot name="top"></slot>
-            <div class="horizontal-wrapper">
+            <div class="horizontal-wrapper" part="container">
               <slot name="start"></slot>
               <slot name="icon-only"></slot>
               <slot></slot>
diff --git a/core/src/components/item-option/test/custom/item-option.e2e.ts b/core/src/components/item-option/test/custom/item-option.e2e.ts
new file mode 100644
index 00000000000..678f1c9d523
--- /dev/null
+++ b/core/src/components/item-option/test/custom/item-option.e2e.ts
@@ -0,0 +1,80 @@
+import { expect } from '@playwright/test';
+import { configs, test } from '@utils/test/playwright';
+
+/**
+ * This behavior does not vary across modes/directions
+ */
+configs({ directions: ['ltr'], modes: ['md'] }).forEach(({ title, config }) => {
+  test.describe(title('item-option: custom'), () => {
+    test.describe(title('CSS shadow parts'), () => {
+      test('should be able to customize native part', async ({ page }) => {
+        await page.setContent(
+          `
+          <style>
+            ion-item-option::part(native) {
+              background-color: red;
+            }
+          </style>
+
+          <ion-item-option>Option</ion-item-option>
+        `,
+          config
+        );
+
+        const itemOption = page.locator('ion-item-option');
+        const backgroundColor = await itemOption.evaluate((el) => {
+          const shadowRoot = el.shadowRoot;
+          const native = shadowRoot?.querySelector('.button-native');
+          return native ? window.getComputedStyle(native).backgroundColor : '';
+        });
+        expect(backgroundColor).toBe('rgb(255, 0, 0)');
+      });
+
+      test('should be able to customize inner part', async ({ page }) => {
+        await page.setContent(
+          `
+          <style>
+            ion-item-option::part(inner) {
+              background-color: green;
+            }
+          </style>
+
+          <ion-item-option>Option</ion-item-option>
+        `,
+          config
+        );
+
+        const itemOption = page.locator('ion-item-option');
+        const backgroundColor = await itemOption.evaluate((el) => {
+          const shadowRoot = el.shadowRoot;
+          const inner = shadowRoot?.querySelector('.button-inner');
+          return inner ? window.getComputedStyle(inner).backgroundColor : '';
+        });
+        expect(backgroundColor).toBe('rgb(0, 128, 0)');
+      });
+
+      test('should be able to customize container part', async ({ page }) => {
+        await page.setContent(
+          `
+          <style>
+            ion-item-option::part(container) {
+              background-color: blue;
+            }
+          </style>
+
+          <ion-item-option>Option</ion-item-option>
+        `,
+          config
+        );
+
+        const itemOption = page.locator('ion-item-option');
+        const backgroundColor = await itemOption.evaluate((el) => {
+          const shadowRoot = el.shadowRoot;
+          const container = shadowRoot?.querySelector('.horizontal-wrapper');
+          return container ? window.getComputedStyle(container).backgroundColor : '';
+        });
+        expect(backgroundColor).toBe('rgb(0, 0, 255)');
+      });
+    });
+  });
+});