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+---
+title: ReadPlate
+description: Measure absorbance values from photographs of 6-, 12-, 24-, 48-, or 96-well plates.
+categories: [Analysis, Color Processing]
+doi: 10.1002/bmb.21139
+
+name: ReadPlate
+initial-release-date: "2015"
+team-founder: "José María Delfino"
+team-maintainer: "José María Delfino"
+support-status: Active
+source-label: GitHub
+source-url: https://github.com/ghanerka/ReadPlate
+---
+
+ReadPlate is an ImageJ macro for measuring light intensity and calculating
+uncorrected, blank, and blank-corrected absorbance values from a color
+photograph of a multi-well plate. ReadPlate 3.0 supports 6 (3x2), 12 (4x3),
+24 (6x4), 48 (8x6), and 96 (12x8) well plates.
+
+## Installation
+
+ReadPlate is distributed through this Fiji update site:
+
+{% include link-banner url="https://ghanerka.github.io/ReadPlate/" %}
+
+In Fiji, choose {% include bc path='Help|Update...' %}, open **Manage update
+sites**, and enable **ReadPlate**. If the canonical registry still shows the
+older unavailable URL, use **Add Unlisted Site** with the URL above. Apply the
+changes and restart Fiji. The command is then available at {% include bc path='Plugins|ReadPlate 3.0' %}.
+
+The update site installs the macro together with its original HTML
+documentation and sample 96-well plate image.
+
+## Requirements
+
+- ImageJ 1.43h or newer
+- A 24-bit RGB plate image
+
+Version 3.0 was tested with ImageJ 1.53c and Java 8. Before running it, choose
+{% include bc path='Analyze|Set Measurements...' %} and enable Area, Standard
+Deviation, Min & Max Gray Value, Mean Gray Value, Modal Gray Value, and Add to
+Overlay. Set Redirect to None and Decimal Places to 3.
+
+## Use
+
+1. Open a color photograph of a plate whose borders are aligned with the image.
+2. Run {% include bc path='Plugins|ReadPlate 3.0' %}.
+3. Select the plate format.
+4. Draw a center-to-center rectangular selection from the upper-left to the
+   lower-right corner well.
+5. Select the red, green, blue, or gray measurement channel.
+6. Adjust the main and ancillary circle-grid parameters, verify the overlay,
+   and collect measurements.
+7. Inspect and optionally save the Results table. Corrected absorbance is
+   reported as `Acorr`.
+
+The full original documentation, macro, sample image, release checksums, and
+citation metadata are available from the [ReadPlate source
+repository](https://github.com/ghanerka/ReadPlate) and its [3.0
+release](https://github.com/ghanerka/ReadPlate/releases/tag/v3.0.0).
+
+## Citation
+
+Angelani CR, Carabias P, Cruz KM, Delfino JM, *et al.* (2018). “A Metabolic
+Control Analysis Approach to Introduce the Study of Systems in Biochemistry:
+the Glycolytic Pathway in the Red Blood Cell.” *Biochemistry and Molecular
+Biology Education* 46(5): 502–515. [doi:10.1002/bmb.21139](https://doi.org/10.1002/bmb.21139)