diff --git a/easystacks/software.eessi.io/2026.06/eessi-2026.06-eb-5.4.1-2026.1.yml b/easystacks/software.eessi.io/2026.06/eessi-2026.06-eb-5.4.1-2026.1.yml
new file mode 100644
index 0000000000..b5dd0efaa5
--- /dev/null
+++ b/easystacks/software.eessi.io/2026.06/eessi-2026.06-eb-5.4.1-2026.1.yml
@@ -0,0 +1,4 @@
+easyconfigs:
+  - BEDTools-2.31.1-GCC-15.2.0.eb
+  - SAMtools-1.23.1-GCC-15.2.0.eb
+  - GATK-4.6.2.0-GCCcore-15.2.0-Java-25.eb