diff --git a/sdk/storage/azure-storage-blob/azure/storage/blob/_shared_access_signature.py b/sdk/storage/azure-storage-blob/azure/storage/blob/_shared_access_signature.py
index 46cb94f9c886..fcb8b3012279 100644
--- a/sdk/storage/azure-storage-blob/azure/storage/blob/_shared_access_signature.py
+++ b/sdk/storage/azure-storage-blob/azure/storage/blob/_shared_access_signature.py
@@ -351,6 +351,10 @@ def add_resource_signature(
         if path[0] != "/":
             path = "/" + path
 
+        # Normalize backslashes to forward slashes to match Azure Storage service behavior
+        # Go and .NET SDKs already perform this normalization
+        path = path.replace("\\", "/")
+
         canonicalized_resource = "/blob/" + account_name + path + "\n"
 
         # Form the string to sign from shared_access_policy and canonicalized
diff --git a/sdk/storage/azure-storage-blob/tests/test_shared_access_signature.py b/sdk/storage/azure-storage-blob/tests/test_shared_access_signature.py
new file mode 100644
index 000000000000..9c2fe73f053b
--- /dev/null
+++ b/sdk/storage/azure-storage-blob/tests/test_shared_access_signature.py
@@ -0,0 +1,19 @@
+from datetime import datetime, timedelta
+
+from azure.storage.blob import BlobSasPermissions, generate_blob_sas
+
+
+def test_generate_blob_sas_normalizes_backslashes_in_canonical_resource():
+    string_to_sign = []
+
+    generate_blob_sas(
+        account_name="account",
+        container_name="container",
+        blob_name="dir\\file",
+        account_key="a2V5",
+        permission=BlobSasPermissions(read=True),
+        expiry=datetime.utcnow() + timedelta(hours=1),
+        sts_hook=string_to_sign.append,
+    )
+
+    assert "/blob/account/container/dir/file\n" in string_to_sign[0]